EFHB Polyclonal antibody

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  IHC staining of mouse testis using 17352-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17352-1-AP (EFHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 17352-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 17352-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EFHB Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 17352-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

CFAP21, Cilia- and flagella-associated protein 21, EF-hand domain-containing family member B

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17352-1-AP targets EFHB in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFHB fusion protein Ag11431
• Full Name: EF-hand domain family, member B
• Calculated Molecular Weight: 669aa,76 kDa; 831aa,94 kDa
• GenBank Accession Number: BC028198
• Gene Symbol: EFHB
• Gene ID (NCBI): 151651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N7U6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFHB (EF-hand domain family member B, also known as CFAP21) interacts with Orai1 in a Ca2+-dependent manner. Human EFHB is an 833-amino acid cytosolic protein with two EF-hand domains between amino acids 561-596 and 597-632 (PMID: 34439314).