Tested Applications
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27141-1-AP targets EGF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25997 Product name: Recombinant human EGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1054-1207 aa of BC093731 Sequence: AHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ Predict reactive species |
| Full Name | epidermal growth factor (beta-urogastrone) |
| Calculated Molecular Weight | 1207 aa, 134 kDa |
| GenBank Accession Number | BC093731 |
| Gene Symbol | EGF |
| Gene ID (NCBI) | 1950 |
| RRID | AB_2880771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Epidermal Growth Factor (EGF) is a type of mitogenic factor that stimulates the proliferation of various cells, including epithelial cells and fibroblasts. It is a small peptide composed of 53 amino acids with a molecular weight of approximately 6,000 Daltons. EGF contains three disulfide bonds, which contribute to its stability under acidic and high-temperature conditions. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF plays a crucial role in regulating cell growth, proliferation, and differentiation. When EGF binds to its receptor (EGFR), it activates intracellular signaling pathways that lead to DNA synthesis and cell proliferation. EGF is naturally present in various tissues and body fluids, including saliva, urine, and milk. It is primarily synthesized in the submandibular glands and duodenum.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EGF antibody 27141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-EGF (cat# 27141-1-AP) has 14 citations across 13 journals including ACS Appl Mater Interfaces, Sci Rep, Pharmaceutics, J Ethnopharmacol, eLife, Cell Systems, PLoS One, Chem Biol Drug Des, and Int J Exp Pathol. Research fields span wound healing, lung cancer, oral squamous cell carcinoma, prostate cancer, gastric cancer, breast cancer, endocervical adenocarcinoma, lupus nephritis, and single-cell communication analysis.
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Paper/PMMA Hybrid 3D Cell Culture Microfluidic Platform for the Study of Cellular Crosstalk | ||
J Ethnopharmacol Proteomics analysis of tumor exosomes reveals vital pathways of Jinfukang inhibiting circulating tumor cells metastasis in lung cancer. | ||
Sci Rep Shikonin inhibits multiple tumor malignant phenotypes and is associated with Hedgehog pathway downregulation in lung adenocarcinoma. | ||
Sci Rep Integrated bioinformatics approaches and expression assays identified a potential prognostic biomarker in oral squamous cell carcinoma. | ||
Chem Biol Drug Des An Integrative Methylation-Metabolism Gene Signature Defines Prognosis and Immunosuppressive Microenvironment in Prostate Cancer. |
