|Positive WB detected in||A431 cells, HeLa cells, L02 cells|
|Positive IP detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
51071-2-AP targets EGFR (C-terminal) in WB, IP,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)|
|Calculated molecular weight||1210 aa, 134 kDa|
|Observed molecular weight||170 kDa|
|GenBank accession number||BC094761|
|Gene ID (NCBI)||1956|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.