|Positive WB detected in||HCT 116 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, rat heart tissue|
|Positive IHC detected in||human colon tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19886-1-AP is PHD2/EGLN1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PHD2/EGLN1 fusion protein Ag13706|
|Full Name||egl nine homolog 1 (C. elegans)|
|Calculated molecular weight||426 aa, 46 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC005369|
|Gene ID (NCBI)||54583|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.
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The DpdtbA induced EMT inhibition in gastric cancer cell lines was through ferritinophagy-mediated activation of p53 and PHD2/hif-1α pathway.
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