Tested Applications
| Positive WB detected in | L02 cells, HCT 116 cells, HEK-293 cells, HEK-293T cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19886-1-AP targets PHD2/EGLN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13706 Product name: Recombinant human EGLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 273-426 aa of BC005369 Sequence: MSSMDDLIRHCNGKLGSYKINGRTKAMVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADIEPKFDRLLFFWSDRRNPHEVQPAYATRYAITVWYFDADERARAKVKYLTGEKGVRVELNKPSDSVGKDVF Predict reactive species |
| Full Name | egl nine homolog 1 (C. elegans) |
| Calculated Molecular Weight | 426 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC005369 |
| Gene Symbol | PHD2/EGLN1 |
| Gene ID (NCBI) | 54583 |
| RRID | AB_10641986 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| IHC protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| IP protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| WB protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHD2/EGLN1 Polyclonal antibody (Cat# 19886-1-AP) has 30 citations. Journals include Cancer Cell, Cell Research, Cell Death & Disease, Advanced Science, EBioMedicine, Developmental Cell, Journal of Investigative Dermatology, Cell Signaling, Bioactive Materials, Frontiers in Immunology, and others. Research fields span oncology (colorectal, ovarian, liver, gastric, cervical cancers), hypoxia/HIF-1α signaling, metabolism, inflammation, renal disease, stroke, and wound repair, with applications mainly in WB, IP, and IHC.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting P4HA1 promotes CD8+ T cell progenitor expansion toward immune memory and systemic anti-tumor immunity | ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation
| ||
J Nanobiotechnology Curcuma longa-derived extracellular vesicle-like particles ameliorate retinal neovascularization through HIF-1α and NRF2 signaling.
| ||
Adv Sci (Weinh) Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway | ||
Cell Death Dis Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. | ||
Cell Mol Biol Lett Immunodynamic axis of fibroblast-driven neutrophil infiltration in acute pancreatitis: NF-κB-HIF-1α-CXCL1 |
Reviews
What customers say
The product received one review with a 4-star rating. The reviewer used it for Western Blot at a 1:1500 dilution on liver tissue and reported it performed well for WB applications. Overall, the feedback is positive, indicating reliable performance for Western Blot use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-13-2025) | good for wb
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