PHD2/EGLN1 Polyclonal antibody

PHD2/EGLN1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19886-1-AP

Synonyms

C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PNAS 137, SM 20, SM20, ZMYND6



Tested Applications

Positive WB detected inHCT 116 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected inhuman colon tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19886-1-AP is PHD2/EGLN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHD2/EGLN1 fusion protein Ag13706
Full Name egl nine homolog 1 (C. elegans)
Calculated molecular weight 426 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC005369
Gene symbol EGLN1
Gene ID (NCBI) 54583
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.

Protocols

Product Specific Protocols
WB protocol for PHD2/EGLN1 antibody 19886-1-APDownload protocol
IHC protocol for PHD2/EGLN1 antibody 19886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Antitumor effects of aconitine in A2780 cells via estrogen receptor β‑mediated apoptosis, DNA damage and migration.

Authors - Xiuying Wang
humanWB

J Inorg Biochem

The DpdtbA induced EMT inhibition in gastric cancer cell lines was through ferritinophagy-mediated activation of p53 and PHD2/hif-1α pathway.

Authors - Deng Guan
  • KD Validated
humanWB

Sci Rep

Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein.

Authors - Lucia Cilenti