PHD1 Polyclonal antibody

PHD1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF

Conjugate

Unconjugated

Cat no : 12984-1-AP

Synonyms

Egl nine homolog 2, EGLN2, EIT6, Estrogen induced tag 6, HIF PH1, HIF prolyl hydroxylase 1, HIFPH1, HPH 1, HPH 3, PHD1



Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, HeLa cells, human spleen tissue, mouse heart tissue, PC-3 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

12984-1-AP targets PHD1 in WB, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHD1 fusion protein Ag3616
Full Name egl nine homolog 2 (C. elegans)
Calculated molecular weight 407 aa, 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC036051
Gene symbol EGLN2
Gene ID (NCBI) 112398
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.

Protocols

Product Specific Protocols
WB protocol for PHD1 antibody 12984-1-APDownload protocol
IF protocol for PHD1 antibody 12984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Leuk Lymphoma

Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.

Authors - Rintaro Ohe
ratIF

J Cell Mol Med

LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation.

Authors - Suping Li