Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, PC-3 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55117-1-AP targets EGR1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | early growth response 1 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | NM_001964 |
| Gene Symbol | EGR1 |
| Gene ID (NCBI) | 1958 |
| RRID | AB_2881272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Early growth response 1 (EGR1) is a member of EGR family which are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation. Western blotting of nuclear and cytoplasmic fractions of CNS tissue verified the cytoplasmic Egr1 as 110-kDa dimer or after denaturation as a 57-kDa version, which is reflecting its theoretical molecular weight of 57 kDa. In the nucleus Egr1 is found as 75-kDa homo- or heterodimer and as 35-kDa variant under strong denaturation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EGR1 antibody 55117-1-AP | Download protocol |
| IF protocol for EGR1 antibody 55117-1-AP | Download protocol |
| IHC protocol for EGR1 antibody 55117-1-AP | Download protocol |
| WB protocol for EGR1 antibody 55117-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-EGR1 monoclonal antibody (Cat# 55117-1-AP) has 48 total citations published in journals including Nature Communications, Neuron, Journal of Clinical Investigation, Science Advances, Cancer Letters, Developmental Cell, Oncogene, Cell Reports, EMBO Reports, and others. Associated research fields include cardiac fibrosis, neuroscience, oncology, liver and kidney diseases, ferroptosis, epigenetic regulation, inflammation, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Nat Commun IRX2 regulates angiotensin II-induced cardiac fibrosis by transcriptionally activating EGR1 in male mice
| ||
Nat Commun Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
J Clin Invest Podocyte histone deacetylase activity regulates murine and human glomerular diseases. | ||
Sci Adv Termination of convulsion seizures by destabilizing and perturbing seizure memory engrams | ||
Cancer Lett Radiation-induced glioblastoma stem cell-mediated T cell exhaustion via EGR1-Gal3-LAG3 axis in glioblastoma.
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