EGR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EGR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 55117-1-AP

Synonyms

G0S30, EGR-1, EGR 1, Early growth response protein 1, early growth response 1

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Validation Data Gallery

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  WB analysis using 55117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55117-1-AP (EGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 55117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using EGR1 antibody (55117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 55117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using EGR1 antibody (55117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 55117-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human EGR1 (55117-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human placenta tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

55117-1-AP targets EGR1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: early growth response 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_001964
• Gene Symbol: EGR1
• Gene ID (NCBI): 1958
• RRID: AB_2881272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Early growth response 1 (EGR1) is a member of EGR family which are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation. Western blotting of nuclear and cytoplasmic fractions of CNS tissue verified the cytoplasmic Egr1 as 110-kDa dimer or after denaturation as a 57-kDa version, which is reflecting its theoretical molecular weight of 57 kDa. In the nucleus Egr1 is found as 75-kDa homo- or heterodimer and as 35-kDa variant under strong denaturation.

Publications

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
human,mouse	WB,IHC	Oncogene; N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition; Authors - Rui Liu
mouse	WB	Int Immunopharmacol; DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages; Authors - Yan Wang
mouse	WB	Mol Neurobiol; Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.; Authors - Ying Li
human	WB,IF,IHC	Talanta; MnO2-DNAzyme-photosensitizer nanocomposite with AIE characteristic for cell imaging and photodynamic-gene therapy.; Authors - Xudong Wang

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