EGR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EGR2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 13491-1-AP

Synonyms

AT591, CMT1D, CMT4E, EGR 2, EGR2, KROX20, Zinc finger protein Krox 20

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Validation Data Gallery

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  WB analysis of MCF-7 using 13491-1-AP

  WB result of EGR2 antibody (13491-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGR2 transfected MCF-7 cells.

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  WB analysis using 13491-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13491-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13491-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13491-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13491-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13491-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13491-1-AP (EGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, mouse brain tissue, mouse kidney tissue, SH-SY5Y cells, MCF-7 cells, PC-3 cells, HepG2 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

13491-1-AP targets EGR2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EGR2 fusion protein Ag4313
• Full Name: early growth response 2 (Krox-20 homolog, Drosophila)
• Calculated Molecular Weight: 50 aa, 3 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC035625
• Gene Symbol: EGR2
• Gene ID (NCBI): 1959
• RRID: AB_2097177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGR2 is also named as KROX20, belongs to the EGR C2H2-type zinc-finger protein family. As a zinc finger transcription factor, it is observed in both the somata and dendrites of central neurons(PMID: 12706208). EGR2 plays an important role in the transient formation of hindbrain developmental compartments or rhombomeres and is also an important factor in peripheral myelination, maintenance of synaptic plasticity and long-term potentiation(PMID: 7707882, 8619872, 8895453, 16203212, 18280047). Egr2 expression is induced downstream of TCR signaling in NKT precursors and Egr2 is directly connected with the key molecular checkpoints defining NKT lineage commitment and stage progression, suggesting that Egr2 not only induces the early lineage-defining transcription factor PLZF, but also controls the downstream expression of the IL-2Rβ chain(PMID: 22306690). Egr2 is regulated by both soluble and membrane-bound neuregulins(PMID: 16129398, 18803322, 8787758) and its concentration is partially modulated by calcium-dependent events(PMID: 19179536). Egr2/Krox20 is also required for induction of Pmp22 (PMID: 21411665), and is nuclear-localized(PMID: 12706208). On western blotting, the observed brand is around 50 kDa or gives a raise around 63 kDa(PMID: 22792185). This antibody is a rabbit polyclonal antibody raised against a region of human EGR2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; The Lin28/let-7 axis is critical for myelination in the peripheral nervous system.; Authors - Deniz Gökbuget
human	WB	Int J Mol Sci; Functional Characterization of Muscarinic Receptors in Human Schwann Cells.; Authors - Roberta Piovesana
mouse	WB	Sci Rep; Bidens pilosa and its active compound inhibit adipogenesis and lipid accumulation via down-modulation of the C/EBP and PPARγ pathways.; Authors - Yu-Chuan Liang
mouse	WB	Mol Neurobiol; Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.; Authors - Ying Li
rat	WB	ACS Biomater Sci Eng; Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves; Authors - Lei Zhang
human	WB	Front Pharmacol; Comprehensive Analysis of the Potential Immune-Related Biomarker ATG101 that Regulates Apoptosis of Cholangiocarcinoma Cells After Photodynamic Therapy.; Authors - Zi-Jian Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (08-26-2019)

Clear band at around 50kDa in rat sciatic nerves whole cell lysates.Also working with human tissue lysates.An additional band at 63kDa is also observed in some samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat sciatic nerve

EGR2 Antibody Western Blot, validation (1:500 dilution) in Rat sciatic nerve (Cat no:13491-1-AP)