EHD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EHD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (2)

Cat no : 24657-1-AP

Synonyms

Testilin, PAST1, PAST, EH domain-containing protein 1, EH domain containing 1

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Validation Data Gallery

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  WB analysis using 24657-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24657-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 24657-1-AP

  IP result of anti-EHD1 (IP:24657-1-AP, 4ug; Detection:24657-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

• 

  IHC staining of human stomach cancer using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse brain tissue, HeLa cells, mouse testis tissue, rat lung tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

24657-1-AP targets EHD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EHD1 fusion protein Ag18400
• Full Name: EH-domain containing 1
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC104799
• Gene Symbol: EHD1
• Gene ID (NCBI): 10938
• RRID: AB_2879658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EHD1 (Eps15 Homology Domain Containing 1) is a protein that plays a crucial role in endocytic recycling, which is the process by which cells recycle their membrane components. It is one of four paralogs in mammals (EHD1-4) and is involved in several membrane trafficking pathways. EHD1 is particularly well-studied and is known to regulate the recycling of various cell surface receptors back to the cell surface after they have been endocytosed. This process is essential for maintaining the proper distribution of receptors and other membrane proteins, which in turn affects cellular signaling and function. EHD1 has been implicated in the regulation of the transferrin receptor (TfR), major histocompatibility complex (MHC) class I proteins, β1 integrins, and other receptors. It has also been shown to interact with Rab11-FIP2 and is localized to peripheral endosomes, suggesting a role in the transport of receptors from early endosomes to the endocytic recycling compartment (ERC). Furthermore, EHD1 has been linked to dynein motors that drive transport from early endosomes to the ERC via a complex including MICAL-L1 and the collapsin response mediator protein-2 (Crmp2).

Publications

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	WB	Sci Rep; Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells.; Authors - Shaarvari Bhat ; KD Validated
human	WB,IHC,IF	BMC Cancer; PMID: 27411790; Authors - Jing Gao ; KD Validated
human	WB,IF	Biochem Biophys Res Commun; A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis.; Authors - Hiroki Yukimoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on thrombosis study. I use this for mouse platelet Western blot and it works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse platelet

FH

Juan (Verified Customer) (05-02-2019)

It works well with a single band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000-1:2000
• Cell Tissue Type: Hela cells