EHF Polyclonal antibody

• Featured Product
• KD/KO Validated

EHF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27195-1-AP

Synonyms

EHF, ESE 3, ESE3, ESE3B, ESEJ, ets homologous factor, hEHF

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Validation Data Gallery

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  WB analysis of LNCaP using 27195-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27195-1-AP (EHF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 27195-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27195-1-AP (EHF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 1 publications below
• ChIP: See 2 publications below

Product Information

The immunogen of 27195-1-AP is EHF Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EHF fusion protein Ag25885
• Full Name: ets homologous factor
• Calculated molecular weight: 35 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC038995
• Gene symbol: EHF
• Gene ID (NCBI): 26298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,ChIP	Oncogenesis; Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.; Authors - Te Zhang ; KD Validated
human	ChIP	Am J Cancer Res; Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.; Authors - Junqiang Li ; KD Validated