Tested Applications
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55436-1-AP targets EHMT1 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | euchromatic histone-lysine N-methyltransferase 1 |
| Calculated Molecular Weight | 141 kDa |
| Observed Molecular Weight | 150-170 kDa |
| GenBank Accession Number | NM_024757 |
| Gene Symbol | EHMT1 |
| Gene ID (NCBI) | 79813 |
| RRID | AB_3670252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9B1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EHMT1, also known as GLP (G9a-like protein), encodes a histone lysine methyltransferase that controls brown adipose cell fate, adaptive thermogenesis, and glucose homeostasis in vivo (PMID: 24196706). EHMTs, along with LSD1 (an H3K4 demethylase), are components of the REST complex, which silences neuronal pathway genes in non-neuronal cells. EHMT1 is mainly responsible for the monomethylation and dimethylation of histone H3 lysine 9 (H3K9), forming a heteromeric complex with EHMT2 (G9a) in euchromatin (PMID: 34214254, 37663929).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EHMT1 antibody 55436-1-AP | Download protocol |
| IP protocol for EHMT1 antibody 55436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product received one review with a 5-star rating. The reviewer used it for Western Blot at a 1:1000 dilution on HEK cells and described the performance as "great." Overall, the feedback is positive, indicating the antibody performed well in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
aditya (Verified Customer) (03-24-2026) | great
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