EHMT2/G9a Polyclonal antibody

• 

  WB analysis of Jurkat using 11595-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11595-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11595-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

EHMT2/G9a Polyclonal Antibody for WB, ELISA

Cat No. 11595-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BAT8, C6orf30, EHMT2, EHMT2/G9a, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

11595-1-AP targets EHMT2/G9a in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2171

  Product name: Recombinant human EHMT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 652-1001 aa of BC018718

  Sequence: ESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT

• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated Molecular Weight: 1210 aa, 132 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC018718
• Gene Symbol: EHMT2
• Gene ID (NCBI): 10919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96KQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Exp Gerontol; Age-related changes in histone modification in rat gastrocnemius muscle.; Authors - Toshinori Yoshihara
human	WB	Mol Cell Biochem; Histone methyltransferase G9a and H3K9 dimethylation inhibit the self-renewal of glioma cancer stem cells.; Authors - Hong Tao
human	WB	Cell Rep; Branched-Chain Amino Acid Metabolic Reprogramming Orchestrates Drug Resistance to EGFR Tyrosine Kinase Inhibitors.; Authors - Yuetong Wang ; KD Validated