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- KD/KO Validated
EHMT2 Polyclonal antibody
EHMT2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29303-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
IP | See 1 publications below |
Product Information
29303-1-AP targets EHMT2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EHMT2 fusion protein Ag29160 |
Full Name | euchromatic histone-lysine N-methyltransferase 2 |
Calculated molecular weight | 1210 aa, 132 kDa |
Observed molecular weight | 160-180 kDa |
GenBank accession number | BC018718 |
Gene symbol | EHMT2 |
Gene ID (NCBI) | 10919 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for EHMT2 antibody 29303-1-AP | Download protocol |
IHC protocol for EHMT2 antibody 29303-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncogenesis Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.
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Mol Biotechnol Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development
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