EID3 Polyclonal antibody

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• EID3 Polyclonal antibody (17241-1-AP)

EID3 Polyclonal Antibody for WB, ELISA

Cat No. 17241-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

E1A-like inhibitor of differentiation 3, EID 3, EID-1-like inhibitor of differentiation 3, EID-3, EP300-interacting inhibitor of differentiation 3

• 

  WB analysis using 17241-1-AP

  Human testis tissue were subjected to SDS PAGE followed by western blot with 17241-1-AP (EID3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17241-1-AP targets EID3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11163

  Product name: Recombinant human EID3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-333 aa of BC027612

  Sequence: MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEALEEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLSDCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDLSSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRLPILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY

• Full Name: EP300 interacting inhibitor of differentiation 3
• Calculated Molecular Weight: 333 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC027612
• Gene Symbol: EID3
• Gene ID (NCBI): 493861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N140
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EID3 (EP300‑interacting inhibitor of differentiation 3) is a nuclear transcriptional repressor belonging to the EID protein family. It is predominantly expressed in testis and binds to CBP/p300 co‑activators to suppress nuclear‑receptor‑dependent gene transcription and restrain cellular differentiation. As a component of SMC5‑SMC6 complex, it participates in DNA double‑strand‑break repair and telomere homeostasis. Aberrant EID3 overexpression drives tumor‑cell proliferation and correlates with poor clinical outcomes in glioblastoma and colorectal carcinoma. (PMID: 15987788)