EIF1AD Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF1AD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20528-1-AP

Synonyms

EIF1AD, Haponin

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Validation Data Gallery

• 

  WB analysis of K-562 using 20528-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20528-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 20528-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20528-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, HEK-293 cells, HL-60 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20528-1-AP is EIF1AD Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF1AD fusion protein Ag14390
• Full Name: eukaryotic translation initiation factor 1A domain containing
• Calculated Molecular Weight: 165 aa, 19 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC005131
• Gene Symbol: EIF1AD
• Gene ID (NCBI): 84285
• RRID: AB_10693533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; Structural basis for the final steps of human 40S ribosome maturation.; Authors - Michael Ameismeier ; KD Validated
human	WB	Elife; The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.; Authors - Laura Plassart
human	WB	Elife; Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36.; Authors - Jasmin van den Heuvel
human	WB	Life Sci Alliance; CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau; Authors - Juan Carlos Polanco