EIF1AX Polyclonal antibody

EIF1AX Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11649-2-AP

Synonyms

eIF-1A X isoform, eIF1A X isoform, EIF1A, eIF 4C, eIF 1A X isoform

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Validation Data Gallery

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  WB analysis using 11649-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11649-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11649-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11649-2-AP (EIF1AX antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11649-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF1AX antibody (11649-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, HEK-293T cells, Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11649-2-AP targets EIF1AX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF1AX fusion protein Ag2248
• Full Name: eukaryotic translation initiation factor 1A, X-linked
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000793
• Gene Symbol: EIF1AX
• Gene ID (NCBI): 1964
• RRID: AB_2097803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

Publications

Species	Application	Title
human	WB,IHC	Cell Prolif; Transcriptional repression of p21 by EIF1AX promotes the proliferation of breast cancer cells.; Authors - Yuhuan Li