EIF1AY Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF1AY Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11193-1-AP

Synonyms

eIF 1A Y isoform, eIF 4C, EIF1AY

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 11193-1-AP

  WB result of EIF1AY antibody (11193-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF1AY transfected MCF-7 cells.

• 

  WB analysis of MCF-7 using 11193-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11193-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 11193-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11193-1-AP (EIF1AY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells, HEK-293 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11193-1-AP targets EIF1AY in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF1AY fusion protein Ag1680
• Full Name: eukaryotic translation initiation factor 1A, Y-linked
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC005248
• Gene Symbol: EIF1AY
• Gene ID (NCBI): 9086
• RRID: AB_2262159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.