|Positive WB detected in||HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20499-1-AP is EIF2AK1 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF2AK1 fusion protein Ag14236|
|Full Name||eukaryotic translation initiation factor 2-alpha kinase 1|
|Calculated molecular weight||630 aa, 71 kDa|
|Observed molecular weight||71 kDa|
|GenBank accession number||BC006524|
|Gene ID (NCBI)||27102|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
Human NLRP1 is a sensor for double-stranded RNA.
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.