EIF2AK1 Polyclonal antibody

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  WB analysis using 20499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20499-1-AP

  WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 20499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20499-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20499-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

20499-1-AP targets EIF2AK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14236

  Product name: Recombinant human EIF2AK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 281-630 aa of BC006524

  Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 1
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC006524
• Gene Symbol: EIF2AK1
• Gene ID (NCBI): 27102
• RRID: AB_10697665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQI3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
human	WB,CoIP	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
human	WB	Science; Human NLRP1 is a sensor for double-stranded RNA.; Authors - Stefan Bauernfried
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KD Validated
human	WB	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty ; KD Validated
human	WB	Mol Cell; Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.; Authors - Jack Chun-Chieh Hsu