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  • KD/KO Validated

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18244-1-AP

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse thymus tissue, rat heart tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC093676
Gene symbol EIF2AK2
Gene ID (NCBI) 5610
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Protocols

Product Specific Protocols
WB protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IHC protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IF protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IP protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging Cell

PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

Authors - Marion Tible

Cell Mol Immunol

ZBP1 mediates interferon-induced necroptosis.

Authors - Daowei Yang
mouseWB

Cells

PMID: 31159306

Authors - Takayuki Inoue
humanWB

Front Microbiol

PMID: 32082291

Authors - Yingcheng Zheng
humanWB,IF

J Virol

The leader protein of Theiler's virus prevents the activation of PKR by dsRNA.

Authors - Fabian Borghese
  • KD Validated
humanWB

J Virol

Defining the role of stress granules in innate immune suppression by the HSV-1 endoribonuclease VHS.

Authors - Hannah M Burgess

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: Mouse Heart