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  • KD/KO Validated

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18244-1-AP

Synonyms

eIF 2A protein kinase 2, EIF2AK2, EIF2AK2, PKR, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC093676
Gene symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2246451
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IHC protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IF protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IP protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
mouseWB

Nat Neurosci

Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Authors - Brian T Kalish
humanWB

Sci Adv

Temporal control of the integrated stress response by a stochastic molecular switch.

Authors - Philipp Klein
humanWB

Nat Commun

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

Authors - Psylvia Léger
humanWB

J Immunother Cancer

Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment

Authors - Weiwei Lin
WB

Nat Commun

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Authors - Hongtan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart