|Positive WB detected in||HepG2 cells, Jurkat cells, mouse thymus tissue, rat heart tissue, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18244-1-AP targets EIF2AK2, PKR in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse , rat samples.
|Tested Reactivity||human, mouse , rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF2AK2, PKR fusion protein Ag13010|
|Full Name||eukaryotic translation initiation factor 2-alpha kinase 2|
|Calculated molecular weight||551 aa, 62 kDa|
|Observed molecular weight||65-68 kDa|
|GenBank accession number||BC093676|
|Gene ID (NCBI)||5610|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.
|Product Specific Protocols|
|WB protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IHC protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IF protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IP protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (03-12-2020)
Strong and specific signal for both western blot and staining experiments.