EIF2AK2, PKR Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF2AK2, PKR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 18244-1-AP

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, EIF2AK2, PKR, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

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Validation Data Gallery

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  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18244-1-AP

  WB result of EIF2AK2, PKR antibody (18244-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK2, PKR transfected HepG2 cells.

• 

  WB analysis of mouse thymus using 18244-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 18244-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18244-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18244-1-AP

  IP Result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18244-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Calculated molecular weight: 551 aa, 62 kDa
• Observed molecular weight: 65-68 kDa
• GenBank accession number: BC093676
• Gene symbol: EIF2AK2
• Gene ID (NCBI): 5610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Publications

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
mouse	WB	Nat Neurosci; Maternal immune activation in mice disrupts proteostasis in the fetal brain.; Authors - Brian T Kalish
human	WB	Sci Adv; Temporal control of the integrated stress response by a stochastic molecular switch.; Authors - Philipp Klein
human	WB	Nat Commun; NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.; Authors - Psylvia Léger
	WB	Nat Commun; Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.; Authors - Hongtan Wu
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart