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EIF2AK2, PKR Polyclonal antibody
EIF2AK2, PKR Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse , rat
Applications
WB, RIP, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18244-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 23 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
RIP | See 1 publications below |
Product Information
18244-1-AP targets EIF2AK2, PKR in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.
Tested Reactivity | human, mouse , rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EIF2AK2, PKR fusion protein Ag13010 |
Full Name | eukaryotic translation initiation factor 2-alpha kinase 2 |
Calculated molecular weight | 551 aa, 62 kDa |
Observed molecular weight | 65-68 kDa |
GenBank accession number | BC093676 |
Gene symbol | EIF2AK2 |
Gene ID (NCBI) | 5610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.
Protocols
Product Specific Protocols | |
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WB protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
IHC protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
IF protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
IP protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. | ||
Nat Neurosci Maternal immune activation in mice disrupts proteostasis in the fetal brain. | ||
Sci Adv Temporal control of the integrated stress response by a stochastic molecular switch. | ||
Nat Commun NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. | ||
Nat Commun Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. | ||
Autophagy USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (03-12-2020) | Strong and specific signal for both western blot and staining experiments.
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