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  • KD/KO Validated

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse , rat





Cat no : 18244-1-AP


eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse thymus tissue, rat heart tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC093676
Gene symbol EIF2AK2
Gene ID (NCBI) 5610
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.


Product Specific Protocols
WB protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IHC protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IF protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IP protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Aging Cell

PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

Authors - Marion Tible

Cell Mol Immunol

ZBP1 mediates interferon-induced necroptosis.

Authors - Daowei Yang


PMID: 31159306

Authors - Takayuki Inoue

J Mol Neurosci

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Authors - Lei Chang

Ann Transl Med

Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing.

Authors - Yong-Fang Wu

Mediators Inflamm

Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.

Authors - Yong Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart