Validation Data Gallery
|Positive WB detected in||HepG2 cells, Jurkat cells, mouse thymus tissue, rat heart tissue, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18244-1-AP targets EIF2AK2, PKR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse , rat samples.
|Tested Reactivity||human, mouse , rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF2AK2, PKR fusion protein Ag13010|
|Full Name||eukaryotic translation initiation factor 2-alpha kinase 2|
|Calculated molecular weight||551 aa, 62 kDa|
|Observed molecular weight||65-68 kDa|
|GenBank accession number||BC093676|
|Gene ID (NCBI)||5610|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.
|Product Specific Protocols|
|WB protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IHC protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IF protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|IP protocol for EIF2AK2, PKR antibody 18244-1-AP||Download protocol|
|Click here to view our Standard Protocols|
PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.
Cell Mol Immunol
ZBP1 mediates interferon-induced necroptosis.
J Mol Neurosci
Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.
Ann Transl Med
Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing.
Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (03-12-2020)
Strong and specific signal for both western blot and staining experiments.