Tested Applications
| Positive WB detected in | MCF7 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18010-1-AP targets EIF2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12509 Product name: Recombinant human EIF2B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC103763 Sequence: MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa |
| Calculated Molecular Weight | 305 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC103763 |
| Gene Symbol | EIF2B1 |
| Gene ID (NCBI) | 1967 |
| RRID | AB_2261996 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14232 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| IHC protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| IP protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| WB protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF2B1 Polyclonal antibody (Cat# 18010-1-AP) has 13 citations published in journals including Science, Nature Microbiology, Nature Communications, EMBO Journal, mBio, iScience, FASEB Journal, Biochemical Journal, Molecular Biology of the Cell, and eLife. Research fields span the integrated stress response, viral evasion of translation inhibition, colorectal cancer, eIF2B complex structure and assembly, pharmacological modulation of stress responses, and Vanishing White Matter Disease. Applications cited include WB, IF, and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Microbiol Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association. | ||
Nat Commun Specific activation of the integrated stress response uncovers regulation of central carbon metabolism and lipid droplet biogenesis | ||
EMBO J Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer
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