Tested Applications
| Positive WB detected in | A431 cells, mouse liver tissue, HEK-293 cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11034-1-AP targets EIF2B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1502 Product name: Recombinant human EIF2B2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC011750 Sequence: MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC011750 |
| Gene Symbol | EIF2B2 |
| Gene ID (NCBI) | 8892 |
| RRID | AB_2096016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49770 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| IP protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| WB protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-eIF2B antibody (Cat# 11034-1-AP) has 12 total citations published across 9 journals including Science, EMBO Journal, Cell Reports, iScience, Elife, RNA, Biochemical Journal, Molecular Biology of the Cell, and Molecular Reproduction and Development. Research fields span the integrated stress response, eIF2B complex structure and function, translation regulation, Vanishing White Matter Disease, actin cytoskeleton remodeling via phase separation, and proteomic analysis of extracellular vesicles.
| Species | Application | Title |
|---|---|---|
EMBO J Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer
| ||
Cell Rep DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling | ||
iScience eIF2B localization and its regulation during the integrated stress response is cell-type specific | ||
RNA EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response. | ||
Biochem J Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.
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