EIF2B2 Polyclonal antibody

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  WB analysis using 11034-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11034-1-AP

  IP result of anti-EIF2B2 (IP:11034-1-AP, 4ug; Detection:11034-1-AP 1:500) with HEK-293 cells lysate 2000 ug.

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  IHC staining of rat brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

EIF2B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11034-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EIF 2Bbeta, EIF2B, EIF2B2, EIF2BB, S20I15, S20III15

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Tested Applications

• Positive WB detected in: A431 cells, mouse liver tissue, HEK-293 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 3 publications below

Product Information

11034-1-AP targets EIF2B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1502

  Product name: Recombinant human EIF2B2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC011750

  Sequence: MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

• Full Name: eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC011750
• Gene Symbol: EIF2B2
• Gene ID (NCBI): 8892
• RRID: AB_2096016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49770
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Elife; Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.; Authors - Carmela Sidrauski
	WB	Elife; The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.; Authors - Yao Liang Wong
human	WB	Elife; eIF2B conformation and assembly state regulate the integrated stress response.; Authors - Michael Schoof
human	WB	Elife; A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation.; Authors - Morgane Boone
human	WB	RNA; EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response.; Authors - Stephanie L Moon
human	WB	Biochem J; Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.; Authors - Noel C Wortham ; KD Validated