EIF2B2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86578-1-RR (same clone as 86578-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86578-1-RR (EIF2B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 86578-1-RR (same clone as 86578-1-PBS)

  IP result of anti-EIF2B2 (IP:86578-1-RR, 4ug; Detection:86578-1-RR 1:3000) with A431 cells lysate 1000 ug. This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 86578-1-RR (same clone as 86578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86578-1-RR (EIF2B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86578-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86578-1-RR against Human EIF2B2 were performed. The affinity constant is 18.7 nM.

EIF2B2 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 86578-1-PBS

Clone No.251505G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EIF 2Bbeta, EIF2B, EIF2B 2, eIF2B GDP-GTP exchange factor subunit beta, EIF2BB

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Product Information

86578-1-PBS targets EIF2B2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1502

  Product name: Recombinant human EIF2B2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC011750

  Sequence: MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

• Full Name: eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC011750
• Gene Symbol: EIF2B2
• Gene ID (NCBI): 8892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49770
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.