EIF3A Polyclonal antibody

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EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26178-1-AP Copied!

Publications (15)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180

• 

  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 26178-1-AP

  WB result of EIF3A antibody (26178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF3A transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 26178-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF3A antibody (26178-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26178-1-AP targets EIF3A in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22515

  Product name: Recombinant human EIF3A protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1033-1382 aa of BC114429

  Sequence: DEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPGRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

• Full Name: eukaryotic translation initiation factor 3, subunit A
• Calculated Molecular Weight: 1382 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC114429
• Gene Symbol: EIF3A
• Gene ID (NCBI): 8661
• RRID: AB_2880413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14152
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The EIF3A Polyclonal antibody (Cat# 26178-1-AP) has 15 citations across journals including Molecular Cancer, Molecular Cell, Nucleic Acids Research, PNAS, Developmental Cell, Cell Reports, Advanced Science, Brain Research Bulletin, iScience, Virology, Frontiers in Cellular and Infection Microbiology, Journal of Clinical Laboratory Analysis, and OncoTargets and Therapy. Research fields span cancer biology, RNA m⁶A modification, translation regulation, viral infection, stroke, osteoarthritis, neurodegeneration, inflammation, and aging.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Mol Cancer; CircZFR/YTHDF3 axis drives lymph node metastasis in cervical cancer via FASN translation; Authors - Mingyi Zhou
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu
human	WB	Mol Cell; YTHDF1 differentiates the contributing roles of mTORC1 in aging; Authors - Chenzhong Xu
		Nucleic Acids Res; Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression; Authors - Baochao Fan
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
		Adv Sci (Weinh); eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR-Mediated Metabolic Rewiring; Authors - Sonam Mittal