EIF3C Polyclonal antibody

EIF3C Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12733-1-AP

Synonyms

eIF3 p110, EIF3C, EIF3CL, EIF3S8

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Validation Data Gallery

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  WB analysis of K-562 using 12733-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12733-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12733-1-AP

  IP result of anti-EIF3C (IP:12733-1-AP, 4ug; Detection:12733-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human colon cancer using 12733-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12733-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12733-1-AP targets EIF3C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3C fusion protein Ag3438
• Full Name: eukaryotic translation initiation factor 3, subunit C
• Calculated Molecular Weight: 913 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC001571
• Gene Symbol: EIF3C
• Gene ID (NCBI): 8663
• RRID: AB_2096747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression; Authors - Yanan Pi