CoraLite® Plus 488-conjugated EIF3D Monoclonal antibody
EIF3D Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C5A3
Cat no : CL488-66024
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL488-66024 is EIF3D Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF3D fusion protein Ag18092 |
Full Name | eukaryotic translation initiation factor 3, subunit D |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC000328 |
Gene symbol | EIF3D |
Gene ID (NCBI) | 8664 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 EIF3D antibody CL488-66024 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |