EIF3D Monoclonal antibody
EIF3D Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2C5A3
Cat no : 66024-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, A431 cells, HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 66024-1-Ig is EIF3D Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF3D fusion protein Ag18092 |
Full Name | eukaryotic translation initiation factor 3, subunit D |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC000328 |
Gene symbol | EIF3D |
Gene ID (NCBI) | 8664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for EIF3D antibody 66024-1-Ig | Download protocol |
IHC protocol for EIF3D antibody 66024-1-Ig | Download protocol |
IF protocol for EIF3D antibody 66024-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |