CoraLite®594-conjugated EIF3D Monoclonal antibody

EIF3D Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C5A3

Cat no : CL594-66024

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66024-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66024 is EIF3D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EIF3D fusion protein Ag18092
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC000328
Gene symbol EIF3D
Gene ID (NCBI) 8664
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Protocols

Product Specific Protocols
IF protocol for CL594 EIF3D antibody CL594-66024Download protocol
Standard Protocols
Click here to view our Standard Protocols