EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10899-1-AP

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6



Tested Applications

Positive WB detected inrat lymph tissue, A431 cells, HeLa cells, Jurkat cells, mouse pancreas tissue, mouse thymus tissue
Positive IHC detected inhuman pancreas cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10899-1-AP targets EIF3E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3E fusion protein Ag1132
Full Name eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight 445 aa, 52 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC008419
Gene symbol EIF3E
Gene ID (NCBI) 3646
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3E antibody 10899-1-APDownload protocol
IHC protocol for EIF3E antibody 10899-1-APDownload protocol
IF protocol for EIF3E antibody 10899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols