Tested Applications
| Positive WB detected in | A431 cells, mouse pancreas tissue, mouse thymus tissue, rat lymph tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10899-1-AP targets EIF3E in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1132 Product name: Recombinant human EIF3E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-445 aa of BC008419 Sequence: SGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY Predict reactive species |
| Full Name | eukaryotic translation initiation factor 3, subunit E |
| Calculated Molecular Weight | 445 aa, 52 kDa |
| Observed Molecular Weight | 48-52 kDa |
| GenBank Accession Number | BC008419 |
| Gene Symbol | EIF3E |
| Gene ID (NCBI) | 3646 |
| RRID | AB_2096898 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60228 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF3E antibody 10899-1-AP | Download protocol |
| IHC protocol for EIF3E antibody 10899-1-AP | Download protocol |
| IP protocol for EIF3E antibody 10899-1-AP | Download protocol |
| WB protocol for EIF3E antibody 10899-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF3E antibody (Cat# 10899-1-AP) has 9 citations published in journals including Oncogene, Cancer Letters, Aging Cell, Frontiers in Immunology, Cell Signaling, Journal of Virology, Cancer Genomics Proteomics, Cellular and Molecular Biology Letters, and PLoS Biology. Research fields span cancer biology (bladder, lung, endometrial, and cervical), stem cell differentiation, Alzheimer's disease, ferroptosis, virology, and stress granule biology. Applications include WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Cancer Lett N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination | ||
Oncogene HERC4 synergizes with USP16 to promote lung adenocarcinoma growth by augmenting protein translation via stabilizing RPS15. | ||
Aging Cell Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells. | ||
Front Immunol Farrerol inhibits ferroptosis and protects against LPS-induced acute lung injury by targeting the RUNX1/SLC7A11 axis. | ||
Cell Signal TRIM35 inhibits endometrial cancer progression via ubiquitination and degradation of EIF3E. |
