EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10899-1-AP

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6

---

Validation Data Gallery

• 

  WB analysis of rat lymph using 10899-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10899-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10899-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10899-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 10899-1-AP

  IP result of anti-EIF3E(IP:10899-1-AP, 4ug; Detection:10899-1-AP 1:500) with Jurkat cells lysate 2440 ug.

• 

  IHC staining of human pancreas cancer using 10899-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 10899-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using EIF3E antibody 10899-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Tested Applications

• Positive WB detected in: rat lymph tissue, HeLa cells, mouse thymus tissue, mouse pancreas tissue, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10899-1-AP targets EIF3E in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3E fusion protein Ag1132
• Full Name: eukaryotic translation initiation factor 3, subunit E
• Calculated molecular weight: 445 aa, 52 kDa
• Observed molecular weight: 48-52 kDa
• GenBank accession number: BC008419
• Gene symbol: EIF3E
• Gene ID (NCBI): 3646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Aging Cell; Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells.; Authors - Yang You
human	IHC	Cancer Genomics Proteomics; Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate; Authors - MOHAMED E. M. SAEED