EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11352-1-AP

Synonyms

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

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Validation Data Gallery

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  WB analysis of HeLa using 11352-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11352-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11352-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11352-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11352-1-AP

  IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.

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  IHC staining of human breast cancer using 11352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11352-1-AP

  Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 11352-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11352-1-AP targets EIF3E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3E fusion protein Ag1856
• Full Name: eukaryotic translation initiation factor 3, subunit E
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC000734
• Gene Symbol: EIF3E
• Gene ID (NCBI): 3646
• RRID: AB_2277716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.