EIF3I Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85603-2-RR (same clone as 85603-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85603-2-RR (EIF3I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85603-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01969-2

  Cytometric bead array standard curve of MP01969-2, EIF3I Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85603-3-PBS. Detection antibody: 85603-2-PBS. Standard: Ag1847. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01969-1

  Cytometric bead array standard curve of MP01969-1, EIF3I Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85603-1-PBS. Detection antibody: 85603-2-PBS. Standard: Ag1847. Range: 0.781-100 ng/mL.

EIF3I Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85603-2-PBS

Clone No.243040B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

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Product Information

85603-2-PBS targets EIF3I as part of a matched antibody pair:

MP01969-1: 85603-1-PBS capture and 85603-2-PBS detection (validated in Cytometric bead array)

MP01969-2: 85603-3-PBS capture and 85603-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EIF3I fusion protein Ag1847
• Full Name: eukaryotic translation initiation factor 3, subunit I
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000413
• Gene Symbol: EIF3I
• Gene ID (NCBI): 8668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13347
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.