EIF4A2 Polyclonal antibody

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• EIF4A2 Polyclonal antibody (11280-1-AP)

EIF4A2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11280-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ATP-dependent RNA helicase eIF4A-2, BM 010, DDX2B, EC:3.6.4.13, eIF 4A II

• 

  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 11280-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 11280-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 11280-1-AP

  IP result of anti-EIF4A2 (IP:11280-1-AP, 4ug; Detection:11280-1-AP 1:500) with Jurkat cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11280-1-AP (EIF4A2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse ovary tissue, Raji cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11280-1-AP targets EIF4A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1810

  Product name: Recombinant human EIF4A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 108-407 aa of BC015842

  Sequence: APTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI

• Full Name: eukaryotic translation initiation factor 4A, isoform 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC015842
• Gene Symbol: EIF4A2
• Gene ID (NCBI): 1974
• RRID: AB_2097378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14240
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Publications

What published studies show

The EIF4A2 Polyclonal antibody (Cat# 11280-1-AP) has 14 citations published in journals including Molecular Cell, PNAS, Oncogene, Cell Reports, Molecular Therapy Nucleic Acids, Molecular Neurobiology, Stem Cell Reviews and Reports, Journal of Virology, British Journal of Haematology, Molecular Carcinogenesis, Biology of Reproduction, Microbes and Infection, and Research in Veterinary Science. Research fields span RNA translation regulation, cancer biology, neuroscience, cardiology, virology, immunology, and reproductive biology.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu
human	WB,IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB,IF	Oncogene; RBM12 drives PD-L1-mediated immune evasion in hepatocellular carcinoma by increasing JAK1 mRNA translation; Authors - Hexu Han
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
mouse	IF	Cell Rep; Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon.; Authors - Xiaoliang Liu
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen