EIF4A3 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF4A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 17504-1-AP

Synonyms

EC:3.6.4.13, DEAD box protein 48, DDX48, DDX 48, ATP-dependent RNA helicase eIF4A-3

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Validation Data Gallery

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  WB analysis of A549 using 17504-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 17504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17504-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17504-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17504-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17504-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17504-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17504-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17504-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17504-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17504-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17504-1-AP

  IP result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.

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  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17504-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 17504-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, mouse thymus tissue, mouse heart tissue, mouse lung tissue, HEK-293 cells, mouse liver tissue, HeLa cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 36 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below
• RIP: See 15 publications below

Product Information

17504-1-AP targets EIF4A3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4A3 fusion protein Ag11701
• Full Name: eukaryotic translation initiation factor 4A, isoform 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC003662
• Gene Symbol: EIF4A3
• Gene ID (NCBI): 9775
• RRID: AB_2097393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Publications

Species	Application	Title
	WB	J Hematol Oncol; METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation; Authors - Meilin Xue
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
		Cell Res; YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.; Authors - Hailing Shi
human	WB,RIP,CoIP	J Exp Clin Cancer Res; Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression; Authors - Lixia Li
human	IF,WB	Cell Death Differ; eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.; Authors - Despoina Sakellariou ; KD Validated
human	WB	Mol Cancer; Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.; Authors - Huizhe Wu