eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17728-1-AP

Synonyms

EIF4G2, Death-associated protein 5, Death associated protein 5, DAP-5, DAP5

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Validation Data Gallery

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  WB analysis using 17728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17728-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17728-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17728-1-AP

  IP result of anti-eIF4G2/DAP5 (IP:17728-1-AP, 4ug; Detection:17728-1-AP 1:400) with MCF-7 cells lysate 3200ug.

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  IHC staining of human skin cancer using 17728-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17728-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17728-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17728-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17728-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eIF4G2/DAP5 antibody (17728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells, Neuro-2a cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• RIP: See 1 publications below

Product Information

17728-1-AP targets eIF4G2/DAP5 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: eIF4G2/DAP5 fusion protein Ag12125
• Full Name: eukaryotic translation initiation factor 4 gamma, 2
• Calculated Molecular Weight: 907 aa, 102 kDa
• Observed Molecular Weight: 98-102 kDa
• GenBank Accession Number: BC043149
• Gene Symbol: EIF4G2
• Gene ID (NCBI): 1982
• RRID: AB_2095895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	Shock; miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2; Authors - Zirui Jia
human	WB,RIP	J Proteome Res; EIF4G2 Promotes Hepatocellular Carcinoma Progression via IRES-dependent PLEKHA1 Translation Regulation; Authors - Manman Li ; KD Validated