Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13527-1-AP targets EIF5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4404 Product name: Recombinant human EIF5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 871-1220 aa of BC032639 Sequence: TTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVNLAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII Predict reactive species |
| Full Name | eukaryotic translation initiation factor 5B |
| Calculated Molecular Weight | 1220 aa, 139 kDa |
| Observed Molecular Weight | 175 kDa |
| GenBank Accession Number | BC032639 |
| Gene Symbol | EIF5B |
| Gene ID (NCBI) | 9669 |
| RRID | AB_2096200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60841 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF5B antibody 13527-1-AP | Download protocol |
| IHC protocol for EIF5B antibody 13527-1-AP | Download protocol |
| WB protocol for EIF5B antibody 13527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF5B Polyclonal antibody (catalog no. 13527-1-AP) has 10 citations. These appear in journals including Advanced Science, Science Advances, EMBO Journal, Cell & Molecular Life Sciences, Translational Oncology, Journal of Virology, PLoS One, and bioRxiv. Research fields span translation initiation, cuproptosis/disulfidptosis and PD-L1 checkpoint therapy, hepatovirus and Senecavirus A infection, cardiac hypertrophy, non-small cell lung cancer, and ribosome/frameshifting biology. Applications cited are primarily Western blot and immunohistochemistry, in human, mouse, and pig samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Cuproptosis and Disulfidptosis Converge to Empower PD-L1 Checkpoint Therapy via Cadict-Induced PD-L1 Translation. | ||
Sci Adv Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses | ||
Cell Mol Life Sci eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B. | ||
Cell Mol Life Sci USP13-Mediated selective autophagy of eIF5B promotes pathological cardiac hypertrophy. | ||
Transl Oncol Tumor-derived exosomal miR-199b-5p promotes proliferation and epithelial-mesenchymal transition in non-small cell lung cancer by targeting CCNL1. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used the antibody at a 1:2000 dilution for Western Blot on colorectal cancer cells and reported that it produced a very strong signal against EIF5B, indicating high sensitivity and reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bill (Verified Customer) (12-23-2019) | This antibody give every strong signal on EIF5B.
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