ELAC1 Polyclonal antibody

ELAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26639-1-AP

Synonyms

D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1

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Validation Data Gallery

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  WB analysis of mouse liver using 26639-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26639-1-AP (ELAC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26639-1-AP is ELAC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELAC1 fusion protein Ag23939
• Full Name: elaC homolog 1 (E. coli)
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC014624
• Gene Symbol: ELAC1
• Gene ID (NCBI): 55520
• RRID: AB_2880586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.