ELAC2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83468-6-RR (same clone as 83468-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83468-6-RR (same clone as 83468-6-PBS)

  WB result of ELAC2 antibody (83468-6-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELAC2 transfected MCF-7 cells. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83468-6-RR (same clone as 83468-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83468-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

ELAC2 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83468-6-PBS

Clone No.240487B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

240487B6, EC:3.1.26.11, elaC homolog 2 (E. coli), ElaC homolog protein 2, ELC2

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Product Information

83468-6-PBS targets ELAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0105

  Product name: Recombinant human ELAC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 86-385 aa of BC004158

  Sequence: NCGEGVQRLMQEHKLKVARLDNIFLTRMHWSNVGGLSGMILTLKETGLPKCVLSGPPQLEKYLEAIKIFSGPLKGIELAVRPHSAPEYEDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPERSSDSELNENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAAIAPIIAAVKDGKSITHEGREILAEELCTPPDPGAAFVVVECPDESFIQPICENATFQRYQGKADAPVALVVHMAPASVLVDSRYQQWMERFGPDTQHLVLNENCASVHNLRSHKI

• Full Name: elaC homolog 2 (E. coli)
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC004158
• Gene Symbol: ELAC2
• Gene ID (NCBI): 60528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9BQ52
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).