ELF1 Polyclonal antibody

ELF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, chIP, ELISA, EMSA

Conjugate

Unconjugated

Cat no : 22565-1-AP

Synonyms

ETS-related transcription factor Elf-1, E74-like factor 1, E74 like factor 1



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, A431 cells, Jurkat cells, HL-60 cells, mouse thymus tissue
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22565-1-AP targets ELF1 in WB, IHC, IF/ICC, chIP, ELISA, EMSA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELF1 fusion protein Ag14689
Full Name E74-like factor 1 (ets domain transcription factor)
Calculated Molecular Weight 619 aa, 67 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC030507
Gene Symbol ELF1
Gene ID (NCBI) 1997
RRIDAB_2879123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.

Protocols

Product Specific Protocols
WB protocol for ELF1 antibody 22565-1-APDownload protocol
IHC protocol for ELF1 antibody 22565-1-APDownload protocol
IF protocol for ELF1 antibody 22565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanEMSA

J Autoimmun

rs9459874 and rs1012656 in CCR6/FGFR1OP confer susceptibility to primary biliary cholangitis.

Authors - Yuki Hitomi
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang
humanchIP

J Exp Clin Cancer Res

TNFAIP8 promotes AML chemoresistance by activating ERK signaling pathway through interaction with Rac1.

Authors - Yihua Pang
humanIHC

BMC Cancer

Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy

Authors - Bingjie Guan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iru (Verified Customer) (06-18-2020)

There are other non-specific multiple bands as well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells