ELK1 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP02998-1

  The mean ELK1 concentration was determined to be 1,382.52 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 841.80 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 1,161.24 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load and 354.56 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02998-1

  Sandwich ELISA standard curve of MP02998-1, Human ELK1 Recombinant Matched Antibody Pair - PBS only. 87009-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26683. 87009-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

ELK1 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 87009-1-PBS

Clone No.252085F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

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Product Information

87009-1-PBS targets ELK1 as part of a matched antibody pair:

MP02998-1: 87009-1-PBS capture and 87009-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26683

  Product name: Recombinant human ELK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 161-320 aa of BC056150

  Sequence: TFTIQSLQPQPPPHPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEELEVAGERGFVPETTKAEPEVPPQEGVPARLPAVVMDTAGQAGGHAASSPEISQPQKGRKPRDLE

• Full Name: ELK1, member of ETS oncogene family
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC056150
• Gene Symbol: ELK1
• Gene ID (NCBI): 2002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19419
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.