ELK4 Polyclonal antibody

ELK4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14666-1-AP

Synonyms

ELK4, SAP 1, SAP1, SRF accessory protein 1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14666-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14666-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14666-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14666-1-AP (ELK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14666-1-AP targets ELK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELK4 fusion protein Ag6273
• Full Name: ELK4, ETS-domain protein (SRF accessory protein 1)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC063676
• Gene Symbol: ELK4
• Gene ID (NCBI): 2005
• RRID: AB_2262305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELK4, also named as SAP1, SAP-1 and SAP1, belongs to the ETS family. It forms a ternary complex with the serum response factor (SRF). ELK4 requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. It is androgen regulated, involved in promoting cell growth, and highly expressed in a subset of prostate cancer. (PMID:19293179 )

Publications

Species	Application	Title
human	WB	Front Oncol; tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway; Authors - Weiguo Xu
mouse	WB	J Pers Med; Exosomes from Microvascular Endothelial Cells under Mechanical Unloading Inhibit Osteogenic Differentiation via miR-92b-3p/ELK4 Axis; Authors - Xiaoyan Zhang
mouse	WB	Brain Res Bull; ELK4 ameliorates cognitive impairment and neuroinflammation induced by obstructive sleep apnea; Authors - Haiming Yang