Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells, L02 cells, mouse stomach tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12727-1-AP targets ELL2 in WB, IF, IP, CoIP, ChIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3428 Product name: Recombinant human ELL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 341-640 aa of BC028412 Sequence: TNRVPPTLNGHLNPTSEKSAAGLPLPPAAAAIPTPPPLPSTYLPISHPPQIVNSNSNSPSTPEGRGTQDLPVDSFSQNDSIYEDQQDKYTSRTSLETLPPGSVLLKCPKPMEENHSMSHKKSKKKSKKHKEKDQIKKHDIETIEEKEEDLKREEEIAKLNNSSPNSSGGVKEDCTASMEPSAIELPDYLIKYIAIVSYEQRQNYKDDFNAEYDEYRALHARMETVARRFIKLDAQRKRLSPGSKEYQNVHEEVLQEYQKIKQSSPNYHEEKYRCEYLHNKLAHIKRLIGEFDQQQAESWS Predict reactive species |
| Full Name | elongation factor, RNA polymerase II, 2 |
| Calculated Molecular Weight | 640 aa, 72 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC028412 |
| Gene Symbol | ELL2 |
| Gene ID (NCBI) | 22936 |
| RRID | AB_10644131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00472 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998).
Publications
What published studies show
The ELL2 Polyclonal antibody (Cat# 12727-1-AP) has 9 citations published in journals including Cell, Mol Cell, Nat Chem Biol, Cell Death Dis, FASEB J, J Biol Chem, and Front Med (Lausanne). Research fields span transcription elongation, mitochondrial translation in muscle differentiation, DNA damage response, liver regeneration, prostate cancer, immunoglobulin secretion in plasma cells, and osteoarthritis therapeutics. Applications cited include WB, IF, ChIP, CoIP, and CUT&Tag in human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.
| ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Nat Chem Biol Stabilization of AFF1 by PARylation ensures transcriptional restart after DNA damage | ||
Cell Death Dis TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response | ||
FASEB J Androgen receptor splicing variant 7 (ARv7) promotes DNA damage response in prostate cancer cells |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer used it for Western Blot on RWPE1 and PC-3 cells with an LI-COR imaging system at a 1:500 dilution, reporting satisfactory performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Beth (Verified Customer) (10-05-2017) | Using LI-COR imaging system.
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