ELMOD2 Polyclonal antibody

ELMOD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13027-1-AP

Synonyms

9830169G11Rik, ELMOD2

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Validation Data Gallery

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  WB analysis using 13027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13027-1-AP (ELMOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 13027-1-AP

  IP result of anti-ELMOD2 (IP:13027-1-AP, 4ug; Detection:13027-1-AP 1:300) with A549 cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 13027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 13027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13027-1-AP targets ELMOD2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELMOD2 fusion protein Ag3653
• Full Name: ELMO/CED-12 domain containing 2
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC015168
• Gene Symbol: ELMOD2
• Gene ID (NCBI): 255520
• RRID: AB_2099579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.