ELMOD2 Polyclonal antibody

ELMOD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13027-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

9830169G11Rik, ELMOD2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIPIHC

WB analysis using 13027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13027-1-AP (ELMOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of A549 using 13027-1-AP
IP result of anti-ELMOD2 (IP:13027-1-AP, 4ug; Detection:13027-1-AP 1:300) with A549 cells lysate 2000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 13027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 13027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13027-1-AP (ELMOD2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	A549 cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue
Positive IP detected in	A549 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13027-1-AP targets ELMOD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3653 Product name: Recombinant human ELMOD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-293 aa of BC015168 Sequence: ISLWEFFYGHFFRFWMKWLLRQMTGKCELQRIFDTYVGAQRTHRIENSLTYSKNKVLQKATHVVQSEVDKYVDDIMKEKNINPEKDASFKICMKMCLLQITGYKQLYLDVESVRKRPYDSDNLQHEELLMKLWNLLMPTKKLNARISKQWAEIGFQGDDPKTDFRGMGILGLINLVYFSENYTSEAHQILSRSNHPKLGYSYAIVGINLTEMAYSLLKSEALKFHLYNLVPGIPTMEHFHQFYCYLVYEFDKFWFEEEPESIMYFNLYREKFHEKIKGLLLDCNVALTLKV Predict reactive species
Full Name	ELMO/CED-12 domain containing 2
Calculated Molecular Weight	293 aa, 32 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC015168
Gene Symbol	ELMOD2
Gene ID (NCBI)	255520
RRID	AB_2099579
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IZ81
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for ELMOD2 antibody 13027-1-AP	Download protocol
IP protocol for ELMOD2 antibody 13027-1-AP	Download protocol
WB protocol for ELMOD2 antibody 13027-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB,IF	Curr Med Sci ELMOD2 Overexpression Predicts Adverse Outcomes and Regulates Tumor Progression in Gliomas Authors - Rui-Chao Li KD Validated View Article