Elastin Polyclonal antibody

Elastin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15257-1-AP

Synonyms

desmosine, elastin, ELN, SVAS, Tropoelastin, WBS, WS



Tested Applications

Positive WB detected inhuman artery tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lung tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15257-1-AP targets Elastin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Elastin fusion protein Ag7179
Full Name elastin
Calculated molecular weight 68 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC065566
Gene symbol ELN
Gene ID (NCBI) 2006
RRIDAB_2878120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils (PMID: 12082143). Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein (PMID: 15837523). Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.

Protocols

Product Specific Protocols
WB protocol for Elastin antibody 15257-1-APDownload protocol
IHC protocol for Elastin antibody 15257-1-APDownload protocol
IP protocol for Elastin antibody 15257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Respir Cell Mol Biol

Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation.

Authors - Jing Ge
humanWB

Int J Mol Sci

αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis

Authors - Maia Yamila Kohon

J Cell Physiol

Novel insight into the role of clusterin on intraocular pressure regulation by modifying actin polymerization and extracellular matrix remodeling in the trabecular meshwork.

Authors - Avinash Soundararajan
humanWB

Cells

Cathepsin K Regulates Intraocular Pressure by Modulating Extracellular Matrix Remodeling and Actin-Bundling in the Trabecular Meshwork Outflow Pathway.

Authors - Avinash Soundararajan
humanWB

Front Pharmacol

The Extracellular Matrix Enriched With Exosomes for the Treatment on Pulmonary Fibrosis in Mice

Authors - Yanzhen Yu
WB

Am J Physiol Cell Physiol

miR-29c Induction Contributes to Down-Regulation of Vascular Extracellular Matrix Proteins by Glucocorticoids.

Authors - Tsai-Der Chuang