Elastin Polyclonal antibody

Elastin Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15257-1-AP

Publications (35)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ELN, WS, WBS, Tropoelastin, SVAS

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 15257-1-AP
Human artery tissue were subjected to SDS PAGE followed by western blot with 15257-1-AP (Elastin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of human placenta using 15257-1-AP
IP result of anti-Elastin (IP:15257-1-AP, 4ug; Detection:15257-1-AP 1:300) with human placenta tissue lysate 4000 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 15257-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 15257-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 15257-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 15257-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human artery tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human lung tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15257-1-AP targets Elastin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7179 Product name: Recombinant human ELN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 307-658 aa of BC065566 Sequence: GLVPGGPGFGPGVVGVPGAGVPGVGVPGAGIPVVPGAGIPGAAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGIPTYGVGAGGFPGFGVGVGGIPGVAGVPSVGGVPGVGGVPGVGISPEAQAAAAAKAAKYGLVPGVGVAPGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPGIGPGGVAGAAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFRAVPGALAAAKAAKYGAAVPGVLGGLGALGGVGIPGGVVGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPGVGGLGGIPPAAAAKAAKYGVAARPGFGLSPIFPGGACLGKACGRKRK Predict reactive species
Full Name	elastin
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	60-70 kDa
GenBank Accession Number	BC065566
Gene Symbol	Elastin
Gene ID (NCBI)	2006
RRID	AB_2878120
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15502
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils (PMID: 12082143). Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein (PMID: 15837523). Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.

Protocols

Product Specific Protocols
IHC protocol for Elastin antibody 15257-1-AP	Download protocol
IP protocol for Elastin antibody 15257-1-AP	Download protocol
WB protocol for Elastin antibody 15257-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Anti-Elastin antibody (Cat# 15257-1-AP) has 35 citations spanning journals including Neuron, Advanced Science, Journal of Controlled Release, Matter Today Bio, Journal of Investigative Dermatology, American Journal of Physiology, Respiratory Research, iScience, Scientific Reports, and Food Chemistry. Research fields cover pulmonary fibrosis, skin aging and photodamage, cardiovascular disease (aortic aneurysm/dissection), tissue engineering and regeneration, extracellular matrix biology, ophthalmology, and drug discovery.

Species	Application	Title
human	IF	Neuron Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier Authors - Yuan Xie View Article
	WB	Adv Sci (Weinh) Fatty Acid Oxidation-Glycolysis Metabolic Transition Affects ECM Homeostasis in Silica-Induced Pulmonary Fibrosis Authors - Wenqing Sun View Article
	IF	J Control Release Elastin production by mRNA technology for rejuvenation of aged skin. Authors - Yingying Shi View Article
	IF	Mater Today Bio Elastin-like polypeptide and triclosan-modified PCL membrane provides aseptic protection in tissue regeneration. Authors - Xueliang Peng View Article
	WB	Adv Healthc Mater Enhanced Vascular Smooth Muscle Cell and Extracellular Matrix Repair Using a Metal-Organic Framework-Based Co-Delivery System for Abdominal Aortic Aneurysm Therapy Authors - Jiahao Lei View Article
	WB	Food Chem Texture maintenance and degradation mechanism of ice-stored grass carp (Ctenopharyngodon idella): A scope of intramuscular connective tissue Authors - Fang Yang View Article