ELP4 Polyclonal antibody

ELP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12746-1-AP

Synonyms

C11orf19, Elongator complex protein 4, ELP4, hELP4, PAX6 neighbor gene protein, PAX6NEB, PAXNEB

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Validation Data Gallery

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  WB analysis of human cerebellum using 12746-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12746-1-AP (ELP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12746-1-AP (ELP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 12746-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 12746-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human cerebellum tissue, human brain tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12746-1-AP targets ELP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELP4 fusion protein Ag3707
• Full Name: elongation protein 4 homolog (S. cerevisiae)
• Calculated Molecular Weight: 424 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC012514
• Gene Symbol: ELP4
• Gene ID (NCBI): 26610
• RRID: AB_2099745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.