EML2 Polyclonal antibody

EML2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13529-1-AP

Synonyms

ELP70, EMAP 2, EMAP2, EMAPL2, EML2, HuEMAP 2

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Validation Data Gallery

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  WB analysis of mouse heart using 13529-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13529-1-AP (EML2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13529-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13529-1-AP (EML2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13529-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13529-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse stomach tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

13529-1-AP targets EML2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EML2 fusion protein Ag4423
• Full Name: echinoderm microtubule associated protein like 2
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC032630
• Gene Symbol: EML2
• Gene ID (NCBI): 24139
• RRID: AB_2098055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Hum Genet; MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.; Authors - Sandeep Aryal
human	WB,IF	Curr Biol; EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules; Authors - Takashi Hotta