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Anti-Human EMR2/CD312 Rabbit Recombinant Antibody, PBS Only (98260-1-PBS)

Anti-Human EMR2/CD312 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human EMR2/CD312 Rabbit Recombinant Antibody, PBS Only,
Brand: Proteintech

EMR2/CD312 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98260-1-PBS

Clone No. 242042B11

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

EMR2, 242042B11, ADGRE2, Adhesion G protein-coupled receptor E2, CD312

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

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FC experiment of human PBMCs using 98260-1-RR (same clone as 98260-1-PBS)
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98260-1-RR (same clone as 98260-1-PBS)
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11)(red) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11)(red) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98260-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98260-1-RR
Biolayer interferometry (BLl) kinetic assays of 98260-1-RR against Human EMR2/CD312 were performed. The affinity constant is 0.529 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

98260-1-PBS targets EMR2/CD312 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2479 Product name: Recombinant Human EMR2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-260 aa of NM_013447.3 Sequence: QDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINECATLSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCLPGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCE Predict reactive species
Full Name	egf-like module containing, mucin-like, hormone receptor-like 2
Calculated Molecular Weight	90 kDa
GenBank Accession Number	NM_013447.3
Gene Symbol	EMR2
Gene ID (NCBI)	30817
RRID	AB_3745926
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UHX3-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as CD312 or ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).