ENO1/2/3 Recombinant antibody

ENO1/2/3 Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, Mouse, Rat, Pig







Cat no : 81504-1-RR


Alpha enolase, C myc promoter binding protein, ENO1, ENO1/2/3, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81504-1-RR targets ENO1/2/3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENO1/2/3 fusion protein Ag1692
Full Name enolase 1, (alpha)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.


Product Specific Protocols
WB protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
IHC protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
IF protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
Standard Protocols
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