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NSE Polyclonal antibody

NSE Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10149-1-AP

Synonyms

enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE



Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, U-251 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human lung tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

10149-1-AP targets NSE in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity goat, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSE fusion protein Ag0196
Full Name enolase 2 (gamma, neuronal)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC002745
Gene symbol ENO2
Gene ID (NCBI) 2026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Protocols

Product Specific Protocols
WB protocol for NSE antibody 10149-1-APDownload protocol
IHC protocol for NSE antibody 10149-1-APDownload protocol
IP protocol for NSE antibody 10149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Biochem Cell Biol

Identification of chimeric RNAs in human infant brains and their implications in neural differentiation.

Authors - Yue Tang
ratIHC

Biochem Biophys Res Commun

Nell-1 promotes the neural-like differentiation of dental pulp cells.

Authors - Qi Han
goatIF

Cell Cycle

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.

Authors - Zhimin Wang
humanWB

Aging (Albany NY)

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.

Authors - Fang Li
humanIHC

J Mol Histol

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Authors - Lawrence Owusu
humanWB,IF

Neurosci Lett

Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy.

Authors - Huiling Cao