|Positive WB detected in||human brain tissue, mouse brain tissue, U-251 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue, human lung tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
10149-1-AP targets NSE in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NSE fusion protein Ag0196|
|Full Name||enolase 2 (gamma, neuronal)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC002745|
|Gene ID (NCBI)||2026|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
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