ENO3 Monoclonal antibody, PBS Only

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  WB analysis using 68147-1-Ig (same clone as 68147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68147-1-Ig (ENO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68147-1-Ig (same clone as 68147-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ENO3 antibody (68147-1-Ig, Clone: 2C12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

ENO3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68147-1-PBS

Clone No.2C12G3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

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Product Information

68147-1-PBS targets ENO3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9642

  Product name: Recombinant human ENO3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 94-434 aa of BC017249

  Sequence: MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

• Full Name: enolase 3 (beta, muscle)
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC017249
• Gene Symbol: ENO3
• Gene ID (NCBI): 2027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13929
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)