ENOX1 Polyclonal antibody

ENOX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23750-1-AP

Synonyms

cCNOX, CNOX, Constitutive Ecto NOX, ENOX1, PIG38

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Validation Data Gallery

• 

  WB analysis of Jurkat using 23750-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 23750-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 23750-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23750-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23750-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23750-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23750-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 23750-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 23750-1-AP (ENOX1 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 23750-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 23750-1-AP (ENOX1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23750-1-AP is ENOX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENOX1 fusion protein Ag20715
• Full Name: ecto-NOX disulfide-thiol exchanger 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC024178
• Gene Symbol: ENOX1
• Gene ID (NCBI): 55068
• RRID: AB_2879316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.