Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, mouse ovary tissue, PC-3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10423-1-AP targets ENOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0674 Product name: Recombinant human ENOX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-455 aa of BC019254 Sequence: MIQSANSHVRRLVNEKAAHEKDMEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWCKQAEEIRNIHNDELMGIRREEEMEMSDDEIEEMTETKETEESVSQAEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT Predict reactive species |
| Full Name | ecto-NOX disulfide-thiol exchanger 2 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC019254 |
| Gene Symbol | ENOX2 |
| Gene ID (NCBI) | 10495 |
| RRID | AB_2099483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| IHC protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| IP protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| WB protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The tNOX (ENOX2) antibody (Cat# 10423-1-AP) has 11 citations. They appear in journals including Journal of Medicinal Chemistry, Journal of Agricultural and Food Chemistry, International Journal of Molecular Sciences, Cancers, Experimental Cell Research, American Journal of Cancer Research, Cell Biochemistry and Biophysics, Biochemical and Biophysical Research Communications, eLife, and Biochimica et Biophysica Acta. Research fields center on cancer biology—melanoma, oral and gastric cancer—covering apoptosis, autophagy, cell proliferation, migration, and the tNOX-SIRT1 axis, using WB, IHC, and IP applications.
| Species | Application | Title |
|---|---|---|
J Med Chem Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1. | ||
J Agric Food Chem Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo. | ||
Int J Mol Sci Ecto-NOX Disulfide-Thiol Exchanger 2 (ENOX2/tNOX) Is a Potential Prognostic Marker in Primary Malignant Melanoma and May Serve as a Therapeutic Target | ||
Cancers (Basel) Bis(chloroacetamidino)-Derived Heteroarene-Fused Anthraquinones Bind to and Cause Proteasomal Degradation of tNOX, Leading to c-Flip Downregulation and Apoptosis in Oral Cancer Cells | ||
Exp Cell Res Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.
| ||
Am J Cancer Res Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells. |
Reviews
What customers say
The product received a 5-star rating in a Western Blot application using epithelial cells at a 1:2000 dilution. The reviewer performed a WB analysis of Enox2 in membrane fractions, comparing normal versus abnormal epithelial cells, with molecular weight markers indicated above each lane. The results demonstrated clear and reliable detection, confirming the antibody's performance in membrane fraction analysis.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wassim (Verified Customer) (04-22-2019) | This is a western blot analysis of Enox2 in membrane fractions as seen in normal epithelial cells versus abnormal cells. The molecular weight of fractions is indicated above each lane.
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